SciFlow 1000 Protocols

Quick Manual: Create a Fluorescein Standard Curve

This instruction manual outlines the use of SciFlow 1000 and including fluorescein as a tracer dye to extrapolate concentrations of compounds (concentration vs. time) across linked cell compartments as fluid moves across a channel.

Once a standard curve is generated, a line equation is calculated. This equation allows back calculation of an absolute fluorescein concentration which is then correlated to the starting concentration of a drug or toxicant.

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